| 1. | Study on construction of hepatocyte growth factor expression vector system and stable expression in rat bone marrow stem cells with
基因的逆转录表达载体及体外感染骨髓间充质干细胞的研究 |
| 2. | Usin g a silkworm baculovirus expression vector system ( bevs ) , a large amount of appa phytase was obtained ( up to 7710 per ml hemolymph )
感染五龄家蚕后appa植酸酶的表达量达到平均每毫升血淋巴7710u 。 |
| 3. | In this study , with adeasy vector system , the recombinant adenovirus containing huctla4 - ig gene was constructed
目前，关于基因转移huctla4 - ig诱导同种异体移植物耐受的研究，国外仅有少数实验室开展，国内未见报道。 |
| 4. | Pgem - t vector system , jm109 competent cells , reverse transcription kit and in vitro translation kit were purchased from promega company
Pgem一t载体，感受态细菌jm109 ，反转录试剂盒和体外翻译试剂盒均购自promega公司。 |
| 5. | For desired gene replacement , hyg / cml and str / spc gene cassettes were also incorporated into the new vector system . it is expected that hygwould be integ
为了验证新型yac载体系统的功能，我们将phz621 ~ phz622进行了适当地改造，构建了质粒phz625 。 |
| 6. | For mass production of egf , bombyx mori baculovirus expression vector system ( bevs ) was adopted in this experiment to express recombinant egf
为大量获得egf ，本研究利用家蚕杆状病毒表达系统，以家蚕作为生物反应器，在家蚕细胞和幼虫中表达人表皮生长因子。 |
| 7. | In the same time , comparison principle establishs the relation between complicated vector systems and simple scalar systems , which makes it possible to get stronger results with fewer conditions
同时，比较原理可以建立复杂的向量系统与简单纯量系统之间的关系，这使得在较少条件下得到较强结果成为可能 |
| 8. | Phz621 - phz622 , the new gene replacement vector system , had been constructed through the insertion of the 1 . 0kb and 1 . 4kb flanking sequences of hau3r gene from wild - type s . lividans in the same natural orientation
预期在携带有大插入片段的基因置换yac分子进入野生型变铅青链霉菌后， yac分子将通过1 . 4kb或1 |
| 9. | ( 一 ) foreign genes can be expressed effectively at low cost in silkworm - bmnpv expression vector system ( silkworm bioreactor ) , which is widely noticed in the world . numerous useful proteins have been expressed and the expression levels were higher in silkworm - bmnpv expression vector than that of others systems
(一)家蚕bmnpv表达载体系统(家蚕生物反应器)作为外源蛋白的低成本高效生产体系日益受到国内外的关注，数百种有用蛋白已在家蚕中获得成功的表达。 |
